Mitat ŞAHİN

Article | 2023 | Polish Journal of Veterinary Sciences26 ( 3 )

Neonatal calf diarrhea (NCD) is one of the most important concerns in cattle production. Escherichia coli is the most important bacterial agent of NCD. Although vaccination and antibiotic treatment are common in NCD, the high antigenic diversity of E. coli and the increase in antibiotic resistance cause difficulties in the control. The study aimed to investigate the rate of E. coli in calf diarrhea, isolate an agent of the NCD E. coli strain, determine antimicrobial resistance, and find out about some surface antigens. Fecal samples (n115) were analyzed to isolate pathogenic E. coli strains with nine mixed infections; sixty-one stra . . .ins isolate from fifty diarrhoeic calves. Among the isolates from diseased animals, 22 K99STaF41, 3 K99STa, 3 strains F41, 2 strains Stx1, one strain K99, one strain eae, and one strain Stx2eae were detected. 27 strains of F17- associated fimbriae have been identified. 17 strains F17a, 6 strains F111, 3 strains F17c, one strain carrying the F17a and F17c gene regions, whereas subfamily typing of one strain could not be performed. Serotypes were determined by molecular and serological methods: 32/61 (52.5) isolates were O101 and 2/61 (3.3) isolates were O9 serotypes. But 27 strain serotypes could not be detected. The antibiotic resistance profiles of the isolates were determined by the disc diffusion method. The resistance rates to antibiotics were trimethoprim- sulphamethoxazole 91.7, ampicillin 86.7, enrofloxacin 86.7, gentamicin 45, tobramycin 41.7, cefotaxime 3.3, and ceftazidime 1.7. Due to increasing antibiotic resistance, prophylaxis is gaining importance. In further research, E. coli surface antigenic structures should be examined in detail, and it should form the basis for vaccine and hyperimmunization studies to be developed. Keyword: antimicrobial susceptibility ; calf diarrhea ; Escherichia coli ; serotype ; virulence facto More less

Ayday CUNUŞOVA

Article | 2020 | Polish Journal of Veterinary Sciences23 ( 2 )

The aim of this study was to evaluate the intestinal and cardiac biomarkers in the determination of intestinal and cardiac damage in dogs with parvoviral enteritis. The material of this study consisted of 10 healthy dogs (control group) and 30 dogs with parvoviral enteritis (experimental group) admitted to the Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University. Serum samples were extracted from the collected blood samples taken from vena cephalica venipuncture for analysis of blood gases, haemogram and to measure the levels of intestinal-fatty acid-binding protein (I-FABP), trefoil factor 3 (TFF-3), . . .claudin-3 (CLDN-3), heart-type fatty acid-binding protein (H-FABP), cardiac troponin I (cTnI), and creatine kinase-myocardial band (CK-MB) by enzyme linked immunosorbent assay (ELISA) test kits. Statistically significant decreases in the blood gas hydrogen ion concentration (pH), partial pressure of oxygen (pO(2)), sodium (Na), bicarbonate (HCO3), and oxygen saturation (SatO(2)) levels and significant increase in the levels of I-FABP, TFF-3, CK-MB, cTnI and also in the haemogram, a decrease in leukocyte (WBC) level and an increase in platelet (THR) level were detected in parvoviral dogs compared to the control group ( More less

Hasan GÜZELBEKTEŞ

Article | 2018 | Polish Journal of Veterinary Sciences21 ( 2 )

The aim of this study was to compare effect of combinations of intravenous isotonic sodium bicarbonate (NaHCO3), acetate Ringer, lactate Ringer and small-volume hypertonic sodium chloride (NaCl) solutions along with oral electrolyte solutions (OES) on the treatment of neonatal calf diarrhea with moderate dehydration and metabolic acidosis. Thirty-two calves with diarrhea were used in the study. Calves were randomly assigned to receive acetate Ringer solution (n=8), lactate Ringer solution (n=8), isotonic NaHCO3 (n=8) and 7.2% saline solutions (n=8), and two liters of OES were administrated to all calves orally at the end of intraven . . .ous administration. Blood samples for blood gas and biochemical analyses were collected at 0 hours and at 0.5, 1, 2, 4, 6 and 24 hours intervals. All the calves had mild to moderate metabolic acidosis on admission. Increased plasma volume and sodium concentration, but decreased serum total protein were observed within 0.5 hours following administration of hypertonic 7.2% NaCl + OES, compared to other 3 groups. In conclusion, administration of intravenous hypertonic 7.2% NaCl solution in small volume along with OES provided fast and effective improvement of dehydration and acid-base abnormalities within short time in treatment of calf diarrhea with moderate dehydration and metabolic acidosis More less